| 质粒名称: | pcDNA3-ATR WT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 31611 |
| 存储实验室: | Aziz Sancar |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | 13347 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ATR / ATM and Rad3 Related |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 8000 bp |
| 启动子: | CMV |
| 标签/融合蛋白: | Flag (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | XhoI (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: |
pcDNA3-ATR WT was a gift from Aziz Sancar (Addgene plasmid # 31611 ; http://n2t.net/addgene:31611 ; RRID:Addgene_31611)
|
| 参考文献部分: | Recruitment of DNA damage checkpoint proteins to damage in transcribed and nontranscribed sequences. Jiang G, Sancar A. Mol Cell Biol. 2006 Jan . 26(1):39-49. 10.1128/MCB.26.1.39-49.2006 PubMed 16354678 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3-ATR WT 13347 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcDNA3-ATR WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13347)
AUTHORS Jiang G, Sancar A
TITLE Recruitment of DNA damage checkpoint proteins to damage in
transcribed and nontranscribed sequences.
JOURNAL Mol Cell Biol. 2006 Jan . 26(1):39-49.
PUBMED 16354678
REFERENCE 2 (bases 1 to 13347)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..13347
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 941..964
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(8938..8955)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8944..9168
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9214..9642
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9301..9320)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9511..9532
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9651..9671)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9656..9985
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9836..9971
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9898..9917
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 10052..10846
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(10106..10125)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 10716..10735
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 11020..11141
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11057..11076)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11111..11130
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11190..11206)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11190..11206)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11203..11225)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11214..11230
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11238..11268)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11283..11304
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11421..11438)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11592..12180)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11672..11691)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12351..13211)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12974..12993
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13212..13316)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccaggatg gactacaagg acgacgatga
961 caagggggaa catggcctgg agctggcttc catgatcccc gccctgcggg agctgggcag
1021 tgccacacca gaggaatata atacagttgt acagaagcca agacaaattc tgtgtcaatt
1081 cattgaccgg atacttacag atgtaaatgt tgttgctgta gaacttgtaa agaaaactga
1141 ctctcagcca acctccgtga tgttgcttga tttcatccag catatcatga aatcctcccc
1201 acttatgttt gtaaatgtga gtggaagcca tgaggccaaa ggcagttgta ttgaattcag
1261 taattggatc ataacgagac ttctgcggat tgcagcaact ccctcctgtc atttgttaca
1321 caagaaaatc tgtgaagtca tctgttcatt attatttctt tttaaaagca agagtcctgc
1381 tatttttggg gtcctcacaa aagaattatt acaacttttt gaagacttgg tttacctcca
1441 tagaagaaat gtgatgggtc atgctgtgga atggccagtg gtcatgagcc gatttttaag
1501 tcaattagat gaacacatgg gatatttaca atcagctcct ttgcagttga tgagtatgca
1561 aaatttagaa tttattgaag tcactttatt aatggttctt actcgtatta ttgcaattgt
1621 gttttttaga aggcaagaac tcttactttg gcagataggt tgtgttctgc tagagtatgg
1681 tagtccaaaa attaaatccc tagcaattag ctttttaaca gaactttttc agcttggagg
1741 actaccagca caaccagcta gcactttttt cagctcattt ttggaattat taaaacacct
1801 tgtagaaatg gatactgacc aattgaaact ctatgaagag ccattatcaa agctgataaa
1861 gacactattt ccctttgaag cagaagctta tagaaatatt gaacctgtct atttaaatat
1921 gctgctggaa aaactctgtg tcatgtttga agacggtgtg ctcatgcggc ttaagtctga
1981 tttgctaaaa gcagctttgt gccatttact gcagtatttc cttaaatttg tgccagctgg
2041 gtatgaatct gctttacaag tcaggaaggt ctatgtgaga aatatttgta aagctctttt
2101 ggatgtgctt ggaattgagg tagatgcaga gtacttgttg ggcccacttt atgcagcttt
2161 gaaaatggaa agtatggaaa tcattgagga gattcaatgc caaactcaac aggaaaacct
2221 cagcagtaat agtgatggaa tatcacccaa aaggcgtcgt ctcagctcgt ctctaaaccc
2281 ttctaaaaga gcaccaaaac agactgagga aattaaacat gtggacatga accaaaagag
2341 catattatgg agtgcactga aacagaaagc tgaatccctt cagatttccc ttgaatacag
2401 tggcctaaag aatcctgtta ttgagatgtt agaaggaatt gctgttgtct tacaactgac
2461 tgctctgtgt actgttcatt gttctcatca aaacatgaac tgccgtactt tcaaggactg
2521 tcaacataaa tccaagaaga aaccttctgt agtgataact tggatgtcat tggattttta
2581 cacaaaagtg cttaagagct gtagaagttt gttagaatct gttcagaaac tggacctgga
2641 ggcaaccatt gataaggtgg tgaaaattta tgatgctttg atttatatgc aagtaaacag
2701 ttcatttgaa gatcatatcc tggaagattt atgtggtatg ctctcacttc catggattta
2761 ttcccattct gatgatggct gtttaaagtt gaccacattt gccgctaatc ttctaacatt
2821 aagctgtagg atttcagata gctattcacc acaggcacaa tcacgatgtg tgtttcttct
2881 gactctgttt ccaagaagaa tattccttga gtggagaaca gcagtttaca actgggccct
2941 gcagagctcc catgaagtaa tccgggctag ttgtgttagt ggatttttta tcttattgca
3001 gcagcagaat tcttgtaaca gagttcccaa gattcttata gataaagtca aagatgattc
3061 tgacattgtc aagaaagaat ttgcttctat acttggtcaa cttgtctgta ctcttcacgg
3121 catgttttat ctgacaagtt ctttaacaga acctttctct gaacacggac atgtggacct
3181 cttctgtagg aacttgaaag ccacttctca acatgaatgt tcatcttctc aactaaaagc
3241 ttctgtctgc aagccattcc ttttcctact gaaaaaaaaa atacctagtc cagtaaaact
3301 tgctttcata gataatctac atcatctttg taagcatctt gattttagag aagatgaaac
3361 agatgtaaaa gcagttcttg gaactttatt aaatttaatg gaagatccag acaaagatgt
3421 tagagtggct tttagtggaa atatcaagca catattggaa tccttggact ctgaagatgg
3481 atttataaag gagctttttg tcttaagaat gaaggaagca tatacacatg cccaaatatc
3541 aagaaataat gagctgaagg ataccttgat tcttacaaca ggggatattg gaagggccgc
3601 aaaaggagat ttggtaccat ttgcactctt acacttattg cattgtttgt tatccaagtc
3661 agcatctgtc tctggagcag catacacaga aattagagct ctggttgcag ctaaaagtgt
3721 taaactgcaa agttttttca gccagtataa gaaacccatc tgtcagtttt tggtagaatc
3781 ccttcactct agtcagatga cagcacttcc gaatactcca tgccagaatg ctgacgtgcg
3841 aaaacaagat gtggctcacc agagagaaat ggctttaaat acgttgtctg aaattgccaa
3901 cgttttcgac tttcctgatc ttaatcgttt tcttactagg acattacaag ttctactacc
3961 tgatcttgct gccaaagcaa gccctgcagc ttctgctctc attcgaactt taggaaaaca
4021 attaaatgtc aatcgtagag agattttaat aaacaacttc aaatatattt tttctcattt
4081 ggtctgttct tgttccaaag atgaattaga acgtgccctt cattatctga agaatgaaac
4141 agaaattgaa ctggggagcc tgttgagaca agatttccaa ggattgcata atgaattatt
4201 gctgcgtatt ggagaacact atcaacaggt ttttaatggt ttgtcaatac ttgcctcatt
4261 tgcatccagt gatgatccat atcagggccc gagagatatc atatcacctg aactgatggc
4321 tgattattta caacccaaat tgttgggcat tttggctttt tttaacatgc agttactgag
4381 ctctagtgtt ggcattgaag ataagaaaat ggccttgaac agtttgatgt ctttgatgaa
4441 gttaatggga cccaaacatg tcagttctgt gagggtgaag atgatgacca cactgagaac
4501 tggccttcga ttcaaggatg attttcctga attgtgttgc agagcttggg actgctttgt
4561 tcgctgcctg gatcatgctt gtctgggctc ccttctcagt catgtaatag tagctttgtt
4621 acctcttata cacatccagc ctaaagaaac tgcagctatc ttccactacc tcataattga
4681 aaacagggat gctgtgcaag attttcttca tgaaatatat tttttacctg atcatccaga
4741 attaaaaaag ataaaagccg ttctccagga atacagaaag gagacctctg agagcactga
4801 tcttcagaca actcttcagc tctctatgaa ggccattcaa catgaaaatg tcgatgttcg
4861 tattcatgct cttacaagct tgaaggaaac cttgtataaa aatcaggaaa aactgataaa
4921 gtatgcaaca gacagtgaaa cagtagaacc tattatctca cagttggtga cagtgctttt
4981 gaaaggttgc caagatgcaa actctcaagc tcggttgctc tgtggggaat gtttagggga
5041 attgggggcg atagatccag gtcgattaga tttctcaaca actgaaactc aaggaaaaga
5101 ttttacattt gtgactggag tagaagattc aagctttgcc tatggattat tgatggagct
5161 aacaagagct taccttgcgt atgctgataa tagccgagct caagattcag ctgcctatgc
5221 cattcaggag ttgctttcta tttatgactg tagagagatg gagaccaacg gcccaggtca
5281 ccaattgtgg aggagatttc ctgagcatgt tcgggaaata ctagaacctc atctaaatac
5341 cagatacaag agttctcaga agtcaaccga ttggtctgga gtaaagaagc caatttactt
5401 aagtaaattg ggtagtaact ttgcagaatg gtcagcatct tgggcaggtt atcttattac
5461 aaaggttcga catgatcttg ccagtaaaat tttcacctgc tgtagcatta tgatgaagca
5521 tgatttcaaa gtgaccatct atcttcttcc acatattctg gtgtatgtct tactgggttg
5581 taatcaagaa gatcagcagg aggtttatgc agaaattatg gcagttctaa agcatgacga
5641 tcagcatacc ataaataccc aagacattgc atctgatctg tgtcaactca gtacacagac
5701 tgtgttctcc atgcttgacc atctcacaca gtgggcaagg cacaaatttc aggcactgaa
5761 agctgagaaa tgtccacaca gcaaatcaaa cagaaataag gtagactcaa tggtatctac
5821 tgtggattat gaagactatc agagtgtaac ccattttcta gacctcatac cccaggatac
5881 tctggcagta gcttcctttc gctccaaagc atacacacga gctgtaatgc actttgaatc
5941 atttattaca gaaaagaagc aaaatattca ggaacatctt ggatttttac agaaattgta
6001 tgctgctatg catgaacctg atggagtggc cggagtcagt gcaattagaa aggcagaacc
6061 atctctaaaa gaacagatcc ttgaacatga aagccttggc ttgctgaggg atgccactgc
6121 ttgttatgac agggctattc agctagaacc agaccagatc attcattatc atggtgtagt
6181 aaagtccatg ttaggtcttg gtcagctgtc tactgttatc actcaggtga atggagtgca
6241 tgctaacagg tccgagtgga cagatgaatt aaacacgtac agagtggaag cagcttggaa
6301 attgtcacag tgggatttgg tggaaaacta tttggcagca gatggaaaat ctacaacatg
6361 gagtgtcaga ctgggacagc tattattatc agccaaaaaa agagatatca cagcttttta
6421 tgactcactg aaactagtga gagcagaaca aattgtacct ctttcagctg caagctttga
6481 aagaggctcc taccaacgag gatatgaata tattgtgaga ttgcacatgt tatgtgagtt
6541 ggagcatagc atcaaaccac ttttccagca ttctccaggt gacagttctc aagaagattc
6601 tctaaactgg gtagctcgac tagaaatgac ccagaattcc tacagagcca aggagcctat
6661 cctggctctc cggagggctt tactaagcct caacaaaaga ccagattaca atgaaatggt
6721 tggagaatgc tggctgcaga gtgccagggt agctagaaag gctggtcacc accagacagc
6781 ctacaatgct ctccttaatg caggggaatc acgactcgct gaactgtacg tggaaagggc
6841 aaagtggctc tggtccaagg gtgatgttca ccaggcacta attgttcttc aaaaaggtgt
6901 tgaattatgt tttcctgaaa atgaaacccc acctgagggt aagaacatgt taatccatgg
6961 tcgagctatg ctactagtgg gccgatttat ggaagaaaca gctaactttg aaagcaatgc
7021 aattatgaaa aaatataagg atgtgaccgc gtgcctgcca gaatgggagg atgggcattt
7081 ttaccttgcc aagtactatg acaaattgat gcccatggtc acagacaaca aaatggaaaa
7141 gcaaggtgat ctcatccggt atatagttct tcattttggc agatctctac aatatggaaa
7201 tcagttcata tatcagtcaa tgccacgaat gttaactcta tggcttgatt atggtacaaa
7261 ggcatatgaa tgggaaaaag ctggccgctc cgatcgtgta caaatgagga atgatttggg
7321 taaaataaac aaggttatca cagagcatac aaactattta gctccatatc aatttttgac
7381 tgctttttca caattgatct ctcgaatttg tcattctcac gatgaagttt ttgttgtctt
7441 gatggaaata atagccaaag tatttctagc ctatcctcaa caagcaatgt ggatgatgac
7501 agctgtgtca aagtcatctt atcccatgcg tgtgaacaga tgcaaggaaa tcctcaataa
7561 agctattcat atgaaaaaat ccttagagaa gtttgttgga gatgcaactc gcctaacaga
7621 taagcttcta gaattgtgca ataaaccggt tgatggaagt agttccacat taagcatgag
7681 cactcatttt aaaatgctta aaaagctggt agaagaagca acatttagtg aaatcctcat
7741 tcctctacaa tcagtcatga tacctacact tccatcaatt ctgggtaccc atgctaacca
7801 tgctagccat gaaccatttc ctggacattg ggcctatatt gcagggtttg atgatatggt
7861 ggaaattctt gcttctcttc agaaaccaaa gaagatttct ttaaaaggct cagatggaaa
7921 gttctacatc atgatgtgta agccaaaaga tgacctgaga aaggattgta gactaatgga
7981 attcaattcc ttgattaata agtgcttaag aaaagatgca gagtctcgta gaagagaact
8041 tcatattcga acatatgcag ttattccact aaatgatgaa tgtgggatta ttgaatgggt
8101 gaacaacact gctggtttga gacctattct gaccaaacta tataaagaaa agggagtgta
8161 tatgacagga aaagaacttc gccagtgtat gctaccaaag tcagcagctt tatctgaaaa
8221 actcaaagta ttccgagaat ttctcctgcc caggcatcct cctatttttc atgagtggtt
8281 tctgagaaca ttccctgatc ctacatcatg gtacagtagt agatcagctt actgccgttc
8341 cactgcagta atgtcaatgg ttggttatat tctggggctt ggagaccgtc atggtgaaaa
8401 tattctcttt gattctttga ctggtgaatg cgtacatgta gatttcaatt gtcttttcaa
8461 taagggagaa acctttgaag ttccagaaat tgtgccattt cgcctgactc ataatatggt
8521 taatggaatg ggtcctatgg gaacagaggg tctttttcga agagcatgtg aagttacaat
8581 gaggctgatg cgtgatcagc gagagccttt aatgagtgtc ttaaagactt ttctacatga
8641 tcctcttgtg gaatggagta aaccagtgaa agggcattcc aaagcgccac tgaatgaaac
8701 tggagaagtt gtcaatgaaa aggccaagac ccatgttctt gacattgagc agcgactaca
8761 aggtgtaatc aagactcgaa atagagtgac aggactgccg ttatctattg aaggacatgt
8821 gcattacctt atacaggaag ctactgatga aaacttacta tgccagatgt atcttggttg
8881 gactccatat atgtgactcg agtctagcta gagggcccgt ttaaacccgc tgatcagcct
8941 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
9001 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
9061 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
9121 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
9181 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
9241 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
9301 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
9361 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
9421 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
9481 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
9541 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
9601 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
9661 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
9721 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
9781 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
9841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
9901 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
9961 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
10021 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
10081 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
10141 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
10201 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
10261 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
10321 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
10381 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
10441 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
10501 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
10561 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
10621 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
10681 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
10741 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
10801 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
10861 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
10921 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
10981 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
11041 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
11101 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
11161 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
11221 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
11281 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
11341 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
11401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
11461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
11521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
11581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
11641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
11701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
11761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
11821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
11881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
11941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
12001 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
12061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
12121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
12181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
12241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
12301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
12361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
12421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
12481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
12541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
12601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
12661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
12721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
12781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
12841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
12901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
12961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
13021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
13081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
13141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
13201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
13261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
13321 tttccccgaa aagtgccacc tgacgtc
//
